> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > ANP32E Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APG01890G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ANP32E antibody (N-term).
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Molecular Weight |
30692 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Nucleus
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Target |
This ANP32E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-44 amino acids from the N-terminal region of human ANP32E.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Histone chaperone that specifically mediates the genome- wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.
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Summary |
Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity (By similarity).
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IF, E
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Dilution |
IF~~1:25
WB~~1:1000
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Synonyms |
Acidic leucine-rich nuclear phosphoprotein 32 family member E, LANP-like protein, LANP-L, ANP32E
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Images |
Fluorescent image of SH-SY5Y cells stained with ANP32E Antibody (N-term)( APG01890G). APG01890G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from SH-SY5Y, U-937 cell line and mouse brain tissue lysate (from left to right), using ANP32E Antibody (N-term) ( APG01890G). APG01890G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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