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ANP32E Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APG01890G 100 μl / $495

ANP32E Antibody (N-term)

Brand

Leading Biology

Catalog Number

APG01890G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ANP32E antibody (N-term).

Molecular Weight

30692 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus

Host

Rabbit

Species Reactivity

Human, Mouse

Target

This ANP32E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-44 amino acids from the N-terminal region of human ANP32E.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Histone chaperone that specifically mediates the genome- wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.

Summary

Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity (By similarity).

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, E

Dilution

IF~~1:25 WB~~1:1000

Synonyms

Acidic leucine-rich nuclear phosphoprotein 32 family member E, LANP-like protein, LANP-L, ANP32E

Images

Fluorescent image of SH-SY5Y cells stained with ANP32E Antibody (N-term)( APG01890G). APG01890G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

Western blot analysis of lysates from SH-SY5Y, U-937 cell line and mouse brain tissue lysate (from left to right), using ANP32E Antibody (N-term) ( APG01890G). APG01890G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Specification

Quantity

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