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BLMH Antibody (Center)

Catalog # Availability Size / Price Inquiry
AMM05619G 100 μl / $495

BLMH Antibody (Center)

Brand

Leading Biology

Catalog Number

AMM05619G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BLMH antibody (Center).

Molecular Weight

52562 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Immunogen

214-242 aa

Target

This BLMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the Central region of human BLMH.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.

Summary

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity).

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, E

Dilution

IF~~1:25 WB~~1:1000

Synonyms

Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH

Images

Fluorescent image of Hela cells stained with BLMH Antibody (Center)( AP20463C). AP20463C was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

BLMH Antibody (Center) (Cat. AMM05619G) western blot analysis in CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the BLMH antibody detected the BLMH protein (arrow).

Specification

Quantity

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