Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > Cleaved LC3A Antibody

Cleaved LC3A Antibody

APR26972G Product Datasheet Protocol

Catalog #	Availability	Size / Price	Inquiry
APR06972G		100 μl / $495

Bulk Orders Inquiry

3 Images

Contact Information

Leading Biology Inc.

tel:1-661-524(LBI)-0262
Info@leadingbiology.com

Show All Distributors

Cleaved LC3A Antibody

Brand	Leading Biology	Catalog Number	APR26972G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Cleaved LC3A antibody.
Molecular Weight	14272 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	89-120 aa
Target	This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A.
Isotype	Rabbit Ig
GeneID	84557
UniProt ID	Q9H492
Function	Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Summary	Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, ICC, E
Dilution	IF~~1:25 IHC-P~~1:10~50 WB~~1:1000 ICC~~1:10~50
Synonyms	Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
Images	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 ( Mouse mouse embryonic fibroblasts cell line) cells labeling Pdx1 with AP1805A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue). Immunofluorescence image showing cytoplasm on NIH/3T3 cell line. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP1805A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Immunofluorescence image showing vesicles staining on Hela cell line. The nuclear counter stain is DAPI (blue). The right image is Hela cells treated with Chloroquine 50μM for 16h. AP1805A staining Cleaved-APG8a in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specification	Product Datasheet Protocol
Quantity

Add to cart Inquiry

Previous Product: ALDH6A1 Polyclonal Antibody Next Product: p53 Antibody (S15)

Best Sellers

Related Products

Select	Brand	Catalog No.	Product Name	Pack Size	Type	Field of Research	Specification	Quantity	Price(USD)
1	Leading Biology	APR03440G	ITGA11 Antibody (N-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
2	Leading Biology	APR04537G	CMIP Antibody (C-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
3	Leading Biology	APR13816G	TRPM5 Antibody	50 μl	Polyclonal Antibodies				$695.00	Add Ask
4	Leading Biology	APR04506G	PCOLCE2 Antibody (Center)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
5	Leading Biology	APR12422G	Human H4 Histamine Receptor (extracellular) Antibody	50 μl	Polyclonal Antibodies				$695.00	Add Ask
6	Leading Biology	APR26894N	ASPH Polyclonal Antibody	100ul	Polyclonal Antibodies	Neuroscience>Calcium Signaling			$495.00	Add Ask