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Hippocalcin Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APG03031G 100 μl / $495

Hippocalcin Antibody (N-term)

Brand

Leading Biology

Catalog Number

APG03031G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Hippocalcin antibody (N-term).

Molecular Weight

22427 Da

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

2-32 aa

Target

This Hippocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human Hippocalcin.

Isotype

Rabbit Ig

Symbol

BDR2

GeneID

UniProt ID

Function

May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds two calcium ions.

Summary

Hippocalcin is a member of neuron-specific calcium-binding proteins family found in the retina and brain. This protein is associated with the plasma membrane. It has similarities to proteins located in the photoreceptor cells that regulate photosignal transduction in a calcium-sensitive manner. This protein displays recoverin activity and a calcium-dependent inhibition of rhodopsin kinase. It is identical to the rat and mouse hippocalcin proteins and thought to play an important role in neurons of the central nervous system in a number of species.

Form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:50~100

Synonyms

Neuron-specific calcium-binding protein hippocalcin, Calcium-binding protein BDR-2, HPCA, BDR2

Images

Western blot analysis of lysate from human brain tissue lysate, using Hippocalcin Antibody (R17)( AP1564A). AP1564A was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human brain tissue reacted with Hippocalcin antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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