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SMAD9 Antibody (Center)

APR10180G Product Datasheet Protocol

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APR10180G		100 μl / $495

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Leading Biology Inc.

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SMAD9 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR10180G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SMAD9 antibody (Center).
Molecular Weight	52493 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Nucleus. Note=In the cytoplasm in the absence of ligand Migration to the nucleus when complexed with SMAD4 (By similarity).
Host	Rabbit
Species Reactivity	Human
Immunogen	200-228 aa
Target	This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9.
Isotype	Rabbit Ig
Symbol	MADH6, MADH9
GeneID	4093
UniProt ID	O15198
Function	Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Summary	The protein encoded by this gene is a member of the SMADfamily, which transduces signals from TGF-beta family members. Theencoded protein is activated by bone morphogenetic proteins andinteracts with SMAD4. Two transcript variants encoding differentisoforms have been found for this gene.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	WB~~1:1000 IF~~1:10~50
Synonyms	Mothers against decapentaplegic homolog 9, MAD homolog 9, Mothers against DPP homolog 9, Madh6, SMAD family member 9, SMAD 9, Smad9, SMAD9, MADH6, MADH9
Images	Anti-SMAD9 Antibody (Center) at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST. SMAD9 Antibody (Center) (Cat. APR10180G) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow). Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center)( APR10180G). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
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