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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > Alkaline Phosphatase (ALPI) Antibody (Center)

Alkaline Phosphatase (ALPI) Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR07058G 100 μl / $495

Alkaline Phosphatase (ALPI) Antibody (Center)

Brand

Leading Biology

Catalog Number

APR07058G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Alkaline Phosphatase (ALPI) antibody (Center).

Molecular Weight

56812 Da

Cellular Localization

Antigen Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor.

Host

Rabbit

Species Reactivity

Human

Immunogen

274-304 aa

Target

This Alkaline Phosphatase (ALPI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI).

Isotype

Rabbit Ig

GeneID

UniProt ID

Summary

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IF, WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:100 IF~~1:100

Synonyms

Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI

Images

Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody (Center)( APR07058G). APR07058G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Immunohistochemical analysis of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody(Center) ( APR07058G). APR07058G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysate from human placenta tissue,using ALPI Antibody (Center)(Cat. APR07058G).APR07058G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.

Specification

Quantity

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