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LPL Antibody (Center)

APR08264G Product Datasheet Protocol

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APR08264G		100 μl / $495

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Leading Biology Inc.

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LPL Antibody (Center)

Brand	Leading Biology	Catalog Number	APR08264G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality LPL antibody (Center).
Molecular Weight	53162 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Locates to the plasma membrane of microvilli of hepatocytes with triacyl- glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity).
Host	Rabbit
Species Reactivity	Human
Immunogen	300-327 aa
Target	This LPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-327 amino acids from the Central region of human LPL.
Isotype	Rabbit Ig
Symbol	LIPD
GeneID	4023
UniProt ID	P06858
Function	The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity).
Summary	LPL encodes lipoprotein lipase, which is expressed inheart, muscle, and adipose tissue. LPL functions as a homodimer,and has the dual functions of triglyceride hydrolase andligand/bridging factor for receptor-mediated lipoprotein uptake.Severe mutations that cause LPL deficiency result in type Ihyperlipoproteinemia, while less extreme mutations in LPL arelinked to many disorders of lipoprotein metabolism. [provided byRefSeq].
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, IHC-P, E
Dilution	FC~~1:25 WB~~1:1000 IHC-P~~1:10~50
Synonyms	Lipoprotein lipase, LPL, LPL, LIPD
Images	Overlay histogram showing Hela cells stained with APR08264G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR08264G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was Rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-LPL Antibody (Center) at 1:1000-1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST. LPL Antibody (Center) (Cat. APR08264G) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the LPL antibody detected the LPL protein (arrow).
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