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TARDBP Antibody (N-term)

APG02984G Product Datasheet Protocol

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APG02984G		100 μl / $495

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Leading Biology Inc.

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TARDBP Antibody (N-term)

Brand	Leading Biology	Catalog Number	APG02984G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TARDBP antibody (N-term).
Molecular Weight	44740 Da
Cellular Localization	Antigen Cellular Localization: Nucleus. Note=In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	1-30 aa
Target	This TARDBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TARDBP.
Isotype	Rabbit Ig
Symbol	TDP43
GeneID	23435
UniProt ID	Q13148
Function	DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
Summary	HIV-1, the causative agent of acquired immunodeficiencysyndrome (AIDS), contains an RNA genome that produces achromosomally integrated DNA during the replicative cycle.Activation of HIV-1 gene expression by the transactivator Tat isdependent on an RNA regulatory element (TAR) located downstream ofthe transcription initiation site. The protein encoded by this geneis a transcriptional repressor that binds to chromosomallyintegrated TAR DNA and represses HIV-1 transcription. In addition,this protein regulates alternate splicing of the CFTR gene. Asimilar pseudogene is present on chromosome 20. [provided byRefSeq].
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50
Synonyms	TAR DNA-binding protein 43, TDP-43, TARDBP, TDP43
Images	All lanes : Anti-TARDBP Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST. TARDBP Antibody (N-term) (Cat. APG02984G) western blot analysis in Hela cell line and mouse brain lysates (35ug/lane).This demonstrates the TARDBP antibody detected the TARDBP protein (arrow). Fluorescent confocal image of Hela cell stained with TARDBP Antibody (N-term)( APG02984G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TARDBP primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
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