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GALNT5 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR16072G 100 μl / $495

GALNT5 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR16072G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GALNT5 antibody (N-term).

Molecular Weight

106266 Da

Cellular Localization

Antigen Cellular Localization: Golgi apparatus membrane; Single-pass type II membrane protein

Host

Rabbit

Species Reactivity

Human

Immunogen

24-53 aa

Target

This GALNT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human GALNT5.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward EA2 peptide substrate, but has a weak activity toward Muc2 or Muc1b substrates (By similarity).

Summary

GALNT5 can catalyze the initial reaction in O-linked oligosaccharide biosynthesis,the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. GALNT5 has activity toward EA2 peptide substrate, but it has a weak activity toward Muc2 or Muc1b substrates.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Polypeptide N-acetylgalactosaminyltransferase 5, Polypeptide GalNAc transferase 5, GalNAc-T5, pp-GaNTase 5, Protein-UDP acetylgalactosaminyltransferase 5, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5, GALNT5

Images

Western blot analysis of lysates from A431, K562 cell line (from left to right), using GALNT5 Antibody (N-term)(Cat. APR16072G). APR16072G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

GALNT5 Antibody (N-term) (Cat. APR16072G) IHC analysis in formalin fixed and paraffin embedded human normal lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GALNT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

GALNT5 Antibody (N-term) (Cat. APR16072G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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