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CLTA Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR11601G 100 μl / $495

CLTA Antibody (Center)

Brand

Leading Biology

Catalog Number

APR11601G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CLTA antibody (Center).

Molecular Weight

27077 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic face of coated pits and vesicles

Host

Rabbit

Species Reactivity

Human

Immunogen

99-127 aa

Target

This CLTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the Central region of human CLTA.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Summary

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Clathrin light chain A, Lca, CLTA

Images

Western blot analysis of CLTA Antibody (Center) (Cat. APR11601G) in MDA-MB231 cell line lysates (35ug/lane). CLTA (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CLTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of CLTA (arrow) using rabbit polyclonal CLTA Antibody (Center) (Cat. APR11601G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTA gene.

Specification

Quantity

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