> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > ATP12A Antibody (Center)Brand |
Leading Biology | Catalog Number |
APR15086G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ATP12A antibody (Center).
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Molecular Weight |
115511 Da
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Cellular Localization |
Antigen Cellular Localization:
Membrane; Multi-pass membrane protein.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
833-859 aa
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Target |
This ATP12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 833-859 amino acids from the Central region of human ATP12A.
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Isotype |
Rabbit Ig
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Symbol |
ATP1AL1
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GeneID |
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UniProt ID |
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Function |
Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues.
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Summary |
ATP12A belongs to the family of P-type cation transport ATPases. This protein is a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Synonyms |
Potassium-transporting ATPase alpha chain 2, Non-gastric H(+)/K(+) ATPase subunit alpha, Proton pump, ATP12A, ATP1AL1
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Images |
Western blot analysis of ATP12A Antibody (Center) (Cat. APR15086G) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ATP12A Antibody (Center)(Cat. APR15086G) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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