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ATP12A Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR15086G 100 μl / $495

ATP12A Antibody (Center)

Brand

Leading Biology

Catalog Number

APR15086G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATP12A antibody (Center).

Molecular Weight

115511 Da

Cellular Localization

Antigen Cellular Localization: Membrane; Multi-pass membrane protein.

Host

Rabbit

Species Reactivity

Human

Immunogen

833-859 aa

Target

This ATP12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 833-859 amino acids from the Central region of human ATP12A.

Isotype

Rabbit Ig

Symbol

ATP1AL1

GeneID

UniProt ID

Function

Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Summary

ATP12A belongs to the family of P-type cation transport ATPases. This protein is a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Potassium-transporting ATPase alpha chain 2, Non-gastric H(+)/K(+) ATPase subunit alpha, Proton pump, ATP12A, ATP1AL1

Images

Western blot analysis of ATP12A Antibody (Center) (Cat. APR15086G) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ATP12A Antibody (Center)(Cat. APR15086G) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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