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AK2 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APG01640G 100 μl / $495

AK2 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APG01640G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality AK2 antibody (C-term).

Molecular Weight

26478 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion intermembrane space.

Host

Rabbit

Species Reactivity

Human

Immunogen

187-217 aa

Target

This AK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-217 amino acids from the C-terminal region of human AK2.

Isotype

Rabbit Ig

Symbol

ADK2

GeneID

UniProt ID

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.

Summary

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:2000 IHC-P~~1:50~100

Synonyms

Adenylate kinase 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03168}, AK 2 {ECO:0000255|HAMAP-Rule:MF_03168}, 2743 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP-AMP transphosphorylase 2 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP:AMP phosphotransferase {ECO:0000255|HAMAP-Rule:MF_03168}, Adenylate monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03168}, Adenylate kinase 2, mitochondrial, N-terminally processed {ECO:0000255|HAMAP-Rule:MF_03168}, AK2 {ECO:0000255|HAMAP-Rule:MF_03168}, ADK2

Images

All lanes : Anti-AK2 Antibody (C-term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-AK2 Antibody (C-term) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specification

Quantity

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