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DGKB Antibody (C-term)

APR07100G Product Datasheet Protocol

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APR07100G		100 μl / $495

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Leading Biology Inc.

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DGKB Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR07100G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DGKB antibody (C-term).
Molecular Weight	90595 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	773-804 aa
Target	This DGKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-804 amino acids from the C-terminal region of human DGKB.
Isotype	Rabbit Ig
Symbol	DAGK2, KIAA0718
GeneID	1607
UniProt ID	Q9Y6T7
Function	Exhibits high phosphorylation activity for long-chain diacylglycerols.
Summary	Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:50~100
Synonyms	Diacylglycerol kinase beta, DAG kinase beta, 90 kDa diacylglycerol kinase, Diglyceride kinase beta, DGK-beta, DGKB, DAGK2, KIAA0718
Images	Western blot analysis of anti-hDGKB-T787 Pab (Cat. APR07100G) in mouse liver tissue lysate. hDGKB-T787(arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. DGKB Antibody (T787) (Cat. APR07100G) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the DGKB antibody detected the DGKB protein (arrow).
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