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PIP5K2G Antibody (C-term)

AMM07197G Product Datasheet Protocol

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AMM07197G		100 μl / $495

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Leading Biology Inc.

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PIP5K2G Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM07197G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PIP5K2G antibody (C-term).
Molecular Weight	47300 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Membrane. Note=Mostly found in the cytosol and surrounding plasma membrane. However, its presence in the endoplasmic reticulum seems to be a prerequisite for PIP2 synthesis (By similarity).
Host	Rabbit
Species Reactivity	Human
Immunogen	333-364 aa
Target	This PIP5K2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-364 amino acids from the C-terminal region of human PIP5K2G.
Isotype	Rabbit Ig
Symbol	PIP5K2C
GeneID	79837
UniProt ID	Q8TBX8
Function	May play an important role in the production of Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic reticulum.
Summary	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:50~100
Synonyms	Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma, Phosphatidylinositol 5-phosphate 4-kinase type II gamma, PI(5)P 4-kinase type II gamma, PIP4KII-gamma, PIP4K2C, PIP5K2C
Images	Western blot analysis of lysate from human brain tissue lysate, using PIP5K2G Antibody(E348)(Cat. AMM07197G). AMM07197G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. Formalin-fixed and paraffin-embedded human testis tissue reacted with PIP5K2G Antibody (C-term) (Cat. AMM07197G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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