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MLK4 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR17393G 100 μl / $495

MLK4 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR17393G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MLK4 antibody (N-term).

Molecular Weight

113957 Da

Host

Rabbit

Species Reactivity

Human

Immunogen

33-62 aa

Target

This MLK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human MLK4.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.

Summary

Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the p38 and JNK signaling cascades. Systematic mutational analyses of tyrosine kinases reveal that a minimum of 30% of colorectal cancer contain at least one mutation in the tyrosine kinases; sampling of colorect cancer cells suggest an important role of MLK4 in this process.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Synonyms

Mitogen-activated protein kinase kinase kinase MLK4, Mixed lineage kinase 4, MLK4 {ECO:0000303|Ref1}

Images

Western blot analysis of lysates from K562, Ramos cell line (from left to right), using MLK4 Antibody (N-term)(Cat. APR17393G). APR17393G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody (N-term) (Cat. APR17393G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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