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Methionyl tRNA Synthetase Antibody (C-term)

APR17354G Product Datasheet Protocol

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APR17354G		100 μl / $495

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Leading Biology Inc.

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Methionyl tRNA Synthetase Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR17354G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Methionyl tRNA Synthetase antibody (C-term).
Molecular Weight	66591 Da
Cellular Localization	Antigen Cellular Localization: Mitochondrion matrix.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	564-593 aa
Target	This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase.
Isotype	Rabbit Ig
GeneID	92935
UniProt ID	Q96GW9
Summary	Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet AMP + diphosphate + L-methionyl-tRNAMetThe 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met).This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:10~50
Synonyms	Methionine--tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2
Images	Anti-MARS2 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of anti-MARS2 Antibody (C-term) (APR17354G) in mouse testis tissue lysates (35ug/lane). MARS2(arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody (C-term) (Cat. APR17354G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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