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ROR1 Antibody

APR13139G Product Datasheet Protocol

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APR13139G		100 μl / $495

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Leading Biology Inc.

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ROR1 Antibody

Brand	Leading Biology	Catalog Number	APR13139G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ROR1 antibody.
Molecular Weight	104283 Da
Cellular Localization	Antigen Cellular Localization: Membrane; Single-pass type I membrane protein
Host	Rabbit
Species Reactivity	Human, Mouse
Target	This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).
Isotype	Rabbit Ig
Symbol	NTRKR1
GeneID	4919
UniProt ID	Q01973
Function	Tyrosine-protein kinase receptor whose role is not yet clear.
Summary	ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 FC~~1:25
Synonyms	Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1, ROR1, NTRKR1
Images	Anti-ROR1 Antibody at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST. APR13139G staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Overlay histogram showing A549 cells stained with APR13139G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR13139G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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