> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > ACO2 Antibody (Center)Brand |
Leading Biology | Catalog Number |
APR07048G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ACO2 antibody (Center).
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Molecular Weight |
85425 Da
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Cellular Localization |
Antigen Cellular Localization:
Mitochondrion.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
294-325 aa
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Target |
This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-325 amino acids from the Central region of human ACO2.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
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Summary |
ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, E
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Dilution |
WB~~1:1000
IHC-P~~1:10~50
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Synonyms |
Aconitate hydratase, mitochondrial, Aconitase, Citrate hydro-lyase, ACO2
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Images |
All lanes : Anti-ACO2 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of ACO2 (arrow) using rabbit polyclonal ACO2 Antibody (Center) (Cat. APR07048G).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACO2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ACO2 antibody (Center) (Cat. APR07048G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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