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ACO2 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR07048G 100 μl / $495

ACO2 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR07048G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ACO2 antibody (Center).

Molecular Weight

85425 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion.

Host

Rabbit

Species Reactivity

Human

Immunogen

294-325 aa

Target

This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-325 amino acids from the Central region of human ACO2.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Summary

ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Synonyms

Aconitate hydratase, mitochondrial, Aconitase, Citrate hydro-lyase, ACO2

Images

All lanes : Anti-ACO2 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of ACO2 (arrow) using rabbit polyclonal ACO2 Antibody (Center) (Cat. APR07048G).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACO2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ACO2 antibody (Center) (Cat. APR07048G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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