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CAPZA1 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR15242G 100 μl / $495

CAPZA1 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR15242G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CAPZA1 antibody (N-term).

Molecular Weight

32923 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm, cytoskeleton.

Host

Rabbit

Species Reactivity

Human

Immunogen

1-30 aa

Target

This CAPZA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CAPZA1.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

Summary

CAPZA1 is a member of the F-actin capping protein alpha subunit family. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, FC, E

Dilution

WB~~1:1000 IF~~1:10~50 FC~~1:10~50

Synonyms

F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1

Images

All lanes : Anti-CAPZA1 Antibody (N-term) at 1:4000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of CAPZA1 antibody (N-term) (Cat. APR15242G) in HL60 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.

Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Specification

Quantity

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