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HIBCH Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR07743G 100 μl / $495

HIBCH Antibody (Center)

Brand

Leading Biology

Catalog Number

APR07743G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HIBCH antibody (Center).

Molecular Weight

43482 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion.

Host

Rabbit

Species Reactivity

Human

Immunogen

183-209 aa

Target

This HIBCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-209 amino acids from the Central region of human HIBCH.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.

Summary

HIBCH is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Synonyms

3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, 3-hydroxyisobutyryl-coenzyme A hydrolase, HIB-CoA hydrolase, HIBYL-CoA-H, HIBCH

Images

Western blot analysis of HIBCH Antibody (Center) (Cat. APR07743G) in K562 cell line lysates (35ug/lane). HIBCH (arrow) was detected using the purified Pab.(2ug/ml)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIBCH antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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