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ANXA2 Antibody (C-term)

APR14961G Product Datasheet Protocol

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APR14961G		100 μl / $495

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Leading Biology Inc.

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ANXA2 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR14961G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ANXA2 antibody (C-term).
Molecular Weight	38604 Da
Cellular Localization	Antigen Cellular Localization: Secreted, extracellular space, extracellular matrix, basement membrane Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism
Host	Rabbit
Species Reactivity	Human
Immunogen	287-313 aa
Target	This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-313 amino acids from the C-terminal region of human ANXA2.
Isotype	Rabbit Ig
Symbol	ANX2, ANX2L4, CAL1H, LPC2D
GeneID	302
UniProt ID	P07355
Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
Summary	ANXA2 is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
Synonyms	Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, PAP-IV, Protein I, p36, ANXA2, ANX2, ANX2L4, CAL1H, LPC2D
Images	Western blot analysis of lysates from A431, SK-BR-3 cell line (from left to right), using ANXA2 Antibody (C-term)(Cat. APR14961G). APR14961G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (C-term) (Cat. APR14961G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Confocal immunofluorescent analysis of ANXA2 Antibody (C-term)( APR14961G) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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