> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > CA5B Antibody (C-term)Brand |
Leading Biology | Catalog Number |
APG02313G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CA5B antibody (C-term).
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Molecular Weight |
36434 Da
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Cellular Localization |
Antigen Cellular Localization:
Mitochondrion.
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
253-283 aa
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Target |
This CA5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-283 amino acids from the C-terminal region of human CA5B.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Reversible hydration of carbon dioxide.
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Summary |
CA5B belongs a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. This protein is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. The protein has a wider tissue distribution than CA VA, which is restricted to the liver.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Synonyms |
Carbonic anhydrase 5B, mitochondrial, Carbonate dehydratase VB, Carbonic anhydrase VB, CA-VB, CA5B
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Images |
Western blot analysis of CA5B Antibody (C-term) (Cat. APG02313G) in mouse liver tissue lysates (35ug/lane). CA5B (arrow) was detected using the purified Pab.
CA5B Antibody (C-term) (Cat. APG02313G) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA5B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
CA5B Antibody (C-term) (Cat. APG02313G) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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