> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > HEXA Antibody (Center)Brand |
Leading Biology | Catalog Number |
APR12368G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality HEXA antibody (Center).
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Molecular Weight |
60703 Da
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Cellular Localization |
Antigen Cellular Localization:
Lysosome.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
315-343 aa
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Target |
This HEXA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-343 amino acids from the Central region of human HEXA.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity.
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Summary |
HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Synonyms |
Beta-hexosaminidase subunit alpha, Beta-N-acetylhexosaminidase subunit alpha, Hexosaminidase subunit A, N-acetyl-beta-glucosaminidase subunit alpha, HEXA
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Images |
Western blot analysis of HEXA Antibody (Center) (Cat. APR12368G) in HepG2 cell line lysates (35ug/lane). HEXA (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HEXA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HEXA Antibody (Center) (Cat. APR12368G) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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