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KCNJ2 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
AMM06108G 100 μl / $495

KCNJ2 Antibody (C-term)

Brand

Leading Biology

Catalog Number

AMM06108G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KCNJ2 antibody (C-term).

Molecular Weight

48288 Da

Cellular Localization

Antigen Cellular Localization: Membrane; Multi-pass membrane protein. Membrane; Lipid-anchor

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

401-427 aa

Target

This KCNJ2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-427 amino acids from the C-terminal region of human KCNJ2.

Isotype

Rabbit Ig

Symbol

IRK1

GeneID

UniProt ID

Function

Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.

Summary

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. This protein is an integral membrane protein and inward-rectifier type potassium channel. This protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Mutations in this gene have been associated with Andersen syndrome, which is racterized by periodic paralysis, cardiac arrhythmias, and dysmorphic features.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Inward rectifier potassium channel 2, Cardiac inward rectifier potassium channel, Inward rectifier K(+) channel Kir21, IRK-1, hIRK1, Potassium channel, inwardly rectifying subfamily J member 2, KCNJ2, IRK1

Images

Anti-KCNJ2 Antibody (C-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of KCNJ2 Antibody (C-term) (Cat. AMM06108G) in mouse lung tissue lysates (35ug/lane). KCNJ2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KCNJ2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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