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SRP72 Antibody (Center)

Catalog # Availability Size / Price Inquiry
AMM07983G 100 μl / $495

SRP72 Antibody (Center)

Brand

Leading Biology

Catalog Number

AMM07983G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SRP72 antibody (Center).

Molecular Weight

74606 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Endoplasmic reticulum

Host

Rabbit

Species Reactivity

Human

Immunogen

119-148 aa

Target

This SRP72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human SRP72.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.

Summary

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. It binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, IHC-P, IF, E

Dilution

WB~~1:1000 FC~~1:10~50 IHC-P~~1:50~100 IF~~1:10~50

Synonyms

Signal recognition particle subunit SRP72, SRP72, Signal recognition particle 72 kDa protein, SRP72

Images

All lanes : Anti-SRP72 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometric analysis of hela cells using SRP72 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human pancreas with SRP72 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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