Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > RASIP1 Antibody (Center)

RASIP1 Antibody (Center)

APR14387G Product Datasheet Protocol

Catalog #	Availability	Size / Price	Inquiry
APR14387G		100 μl / $495

Bulk Orders Inquiry

3 Images

Contact Information

Leading Biology Inc.

tel:1-661-524(LBI)-0262
Info@leadingbiology.com

Show All Distributors

RASIP1 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR14387G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RASIP1 antibody (Center).
Molecular Weight	103457 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, perinuclear region. Golgi apparatus, Golgi stack. Note=Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS
Host	Rabbit
Species Reactivity	Human
Immunogen	651-678 aa
Target	This RASIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 651-678 amino acids from the Central region of human RASIP1.
Isotype	Rabbit Ig
GeneID	54922
UniProt ID	Q5U651
Function	Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS- mediated transformation. Negative regulator of amino acid starvation-induced autophagy.
Summary	RASIP1 may act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, IHC-P, E
Dilution	FC~~1:25 WB~~1:1000 IHC-P~~1:50~100
Synonyms	Ras-interacting protein 1, Rain, RASIP1
Images	Overlay histogram showing K562 cells stained with APR14387G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR14387G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-RASIP1 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of RASIP1 Antibody (Center) (Cat. APR14387G) in K562 cell line lysates (35ug/lane). RASIP1 (arrow) was detected using the purified Pab.
Specification	Product Datasheet Protocol
Quantity

Add to cart Inquiry

Previous Product: ALDH6A1 Polyclonal Antibody Next Product: p53 Antibody (S15)

Best Sellers

Related Products

Select	Brand	Catalog No.	Product Name	Pack Size	Type	Field of Research	Specification	Quantity	Price(USD)
1	Leading Biology	APR03440G	ITGA11 Antibody (N-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
2	Leading Biology	APR04537G	CMIP Antibody (C-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
3	Leading Biology	APR12422G	Human H4 Histamine Receptor (extracellular) Antibody	50 μl	Polyclonal Antibodies				$695.00	Add Ask
4	Leading Biology	APR03844G	UBE2W Antibody (C-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
5	Leading Biology	APR04349G	HECTD2 Antibody (N-term)	100 μl	Polyclonal Antibodies				$495.00	Add Ask
6	Leading Biology	APR03502G	IGHG1 Antibody (Center)	100 μl	Polyclonal Antibodies				$495.00	Add Ask