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ENTH Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR15842G 100 μl / $495

ENTH Antibody (Center)

Brand

Leading Biology

Catalog Number

APR15842G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ENTH antibody (Center).

Molecular Weight

68259 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network

Host

Rabbit

Species Reactivity

Human

Immunogen

222-249 aa

Target

This ENTH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-249 amino acids from the Central region of human ENTH.

Isotype

Rabbit Ig

Symbol

ENTH, EPN4, EPNR, KIAA0171

GeneID

UniProt ID

Function

Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.

Summary

ENTH binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). It May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. It stimulates clathrin assembly.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:50~100

Synonyms

Clathrin interactor 1, Clathrin-interacting protein localized in the trans-Golgi region, Clint, Enthoprotin, Epsin-4, Epsin-related protein, EpsinR, CLINT1, ENTH, EPN4, EPNR, KIAA0171

Images

Western blot analysis of ENTH Antibody (Center) (Cat. APR15842G) in HL-60 cell line lysates (35ug/lane). ENTH (arrow) was detected using the purified Pab.

(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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