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FUCA1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
AMM04633G 100 μl / $495

FUCA1 Antibody (Center)

Brand

Leading Biology

Catalog Number

AMM04633G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality FUCA1 antibody (Center).

Molecular Weight

53689 Da

Cellular Localization

Antigen Cellular Localization: Lysosome.

Host

Rabbit

Species Reactivity

Human

Immunogen

265-294 aa

Target

This FUCA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human FUCA1.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins.

Summary

FUCA1 is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Tissue alpha-L-fucosidase, Alpha-L-fucosidase I, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase 1, FUCA1

Images

Western blot analysis of FUCA1 Antibody (Center) (Cat. AMM04633G) in HepG2 cell line lysates (35ug/lane). FUCA1 (arrow) was detected using the purified Pab.

FUCA1 Antibody (Center) (Cat. AMM04633G) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUCA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

FUCA1 Antibody (Center) (Cat. AMM04633G) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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