> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > SPCS3 Antibody (C-term)Brand |
Leading Biology | Catalog Number |
AMM07954G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SPCS3 antibody (C-term).
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Molecular Weight |
20313 Da
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Cellular Localization |
Antigen Cellular Localization:
Microsome membrane; Single- pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
152-180 aa
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Target |
This SPCS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the C-terminal region of human SPCS3.
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Isotype |
Rabbit Ig
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Symbol |
SPC22
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GeneID |
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UniProt ID |
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Function |
Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.
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Summary |
SPCS3 is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Synonyms |
Signal peptidase complex subunit 3, 34--, Microsomal signal peptidase 22/23 kDa subunit, SPC22/23, SPase 22/23 kDa subunit, SPCS3, SPC22
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Images |
Western blot analysis of SPCS3 antibody (C-term) (Cat. AMM07954G) in mouse liver tissue lysates (35ug/lane). SPCS3 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
SPCS3 Antibody (C-term)(Cat. AMM07954G) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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