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HHAT Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR16688G 100 μl / $495

HHAT Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR16688G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HHAT antibody (N-term).

Molecular Weight

57313 Da

Cellular Localization

Antigen Cellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein

Host

Rabbit

Species Reactivity

Human

Immunogen

30-59 aa

Target

This HHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human HHAT.

Isotype

Rabbit Ig

Symbol

MART2, SKI1

GeneID

UniProt ID

Function

Catalyzes N-terminal palmitoylation of SHH; which is required for SHH signaling (By similarity). May bind GTP.

Summary

'Skinny hedgehog' (SKI1) encodes an enzyme that actswithin the secretory pathway to catalyze amino-terminalpalmitoylation of 'hedgehog' (see MIM 600725).

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Protein-cysteine N-palmitoyltransferase HHAT, 231-, Hedgehog acyltransferase, Melanoma antigen recognized by T-cells 2, MART-2, Skinny hedgehog protein 1, HHAT, MART2, SKI1

Images

HHAT Antibody (N-term) (Cat. APR16688G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HHAT antibody detected the HHAT protein (arrow).

HHAT Antibody (N-term) (Cat. APR16688G) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHAT Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HHAT Antibody (N-term) (Cat. APR16688G) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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