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CYC1 Antibody (C-term)

APG02850G Product Datasheet Protocol

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APG02850G		100 μl / $495

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Leading Biology Inc.

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CYC1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APG02850G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYC1 antibody (C-term).
Molecular Weight	35422 Da
Cellular Localization	Antigen Cellular Localization: Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side
Host	Rabbit
Species Reactivity	Human
Immunogen	259-287 aa
Target	This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the C-terminal region of human CYC1.
Isotype	Rabbit Ig
GeneID	1537
UniProt ID	P08574
Function	This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Summary	This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Synonyms	Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1
Images	CYC1 Antibody (C-term) (Cat. APG02850G) western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).This demonstrates the CYC1 antibody detected the CYC1 protein (arrow). CYC1 Antibody (C-term) (Cat. APG02850G) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYC1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. CYC1 Antibody (C-term) (Cat. APG02850G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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