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G6PC Antibody (Center)

Catalog # Availability Size / Price Inquiry
AMM04640G 100 μl / $495

G6PC Antibody (Center)

Brand

Leading Biology

Catalog Number

AMM04640G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality G6PC antibody (Center).

Molecular Weight

40484 Da

Cellular Localization

Antigen Cellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein

Host

Rabbit

Species Reactivity

Human

Immunogen

123-149 aa

Target

This G6PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-149 amino acids from the Central region of human G6PC.

Isotype

Rabbit Ig

Symbol

G6PT

GeneID

UniProt ID

Function

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.

Summary

Glucose-6-phosphatase is an integral membrane protein of the endoplasmic reticulum that catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate. It is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Defects in the enzyme cause glycogen storage disease type I.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Glucose-6-phosphatase, G-6-Pase, G6Pase, Glucose-6-phosphatase alpha, G6Pase-alpha, G6PC, G6PT

Images

Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of G6PC Antibody (Center) (Cat. AMM04640G) in K562 cell line lysates (35ug/lane).G6PC (arrow) was detected using the purified Pab.

G6PC Antibody (Center) (Cat. AMM04640G) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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