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NSE Antibody

Catalog # Availability Size / Price Inquiry
APR08838G 100 μl / $495

NSE Antibody

Brand

Leading Biology

Catalog Number

APR08838G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NSE antibody.

Molecular Weight

47 KDa

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

392-425 aa

GeneID

UniProt ID

Function

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Summary

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, IHC

Dilution

WB~~ 1:1000 IF~~1:100 IHC~~1:50-1:100

Synonyms

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

Images

Western blot analysis of lysates from U-87 MG,SH-SY5Y cell line (from left to right),using NSE Antibody(C0280). C0280 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Western blot analysis of extracts from Hela cells (Lane 1), colo cells(Lane 2),HepG2 cells(Lane 3) and 293 cells (Lane 4), using NSE Antibody. The lane on the left is treated with synthesized peptide.

Immunofluorescence analysis of A549 cells, using NSE antibody .

Specification

Quantity

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