> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > PDIA3 Antibody (Center)Brand |
Leading Biology | Catalog Number |
AMM07074G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PDIA3 antibody (Center).
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Molecular Weight |
56782 Da
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Cellular Localization |
Antigen Cellular Localization:
Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
192-220 aa
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Target |
This PDIA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the Central region of human PDIA3.
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Isotype |
Rabbit Ig
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Symbol |
ERP57, ERP60, GRP58
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GeneID |
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UniProt ID |
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Summary |
PDIA3 is the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Synonyms |
Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, PDIA3, ERP57, ERP60, GRP58
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Images |
Western blot analysis of PDIA3 Antibody (Center) (Cat. AMM07074G) in A2058, A375, Ramos cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml)
Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center) (Cat. AMM07074G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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