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PDIA3 Antibody (Center)

Catalog # Availability Size / Price Inquiry
AMM07074G 100 μl / $495

PDIA3 Antibody (Center)

Brand

Leading Biology

Catalog Number

AMM07074G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PDIA3 antibody (Center).

Molecular Weight

56782 Da

Cellular Localization

Antigen Cellular Localization: Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Host

Rabbit

Species Reactivity

Human

Immunogen

192-220 aa

Target

This PDIA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the Central region of human PDIA3.

Isotype

Rabbit Ig

Symbol

ERP57, ERP60, GRP58

GeneID

UniProt ID

Summary

PDIA3 is the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, PDIA3, ERP57, ERP60, GRP58

Images

Western blot analysis of PDIA3 Antibody (Center) (Cat. AMM07074G) in A2058, A375, Ramos cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml)

Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center) (Cat. AMM07074G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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