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TPI1 Antibody (N-term)

APR10522G Product Datasheet Protocol

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APR10522G		100 μl / $495

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Leading Biology Inc.

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TPI1 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR10522G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TPI1 antibody (N-term).
Molecular Weight	30791 Da
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	68-96 aa
Target	This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human TPI1.
Isotype	Rabbit Ig
Symbol	TPI
GeneID	7167
UniProt ID	P60174
Summary	TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
Synonyms	Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI
Images	All lanes : Anti-TPI1 Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of TPI1 Antibody (N-term) (Cat. APR10522G) in Y79(lane 1),CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml) Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term) (Cat. APR10522G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.
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