> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > SMPD2 Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APR13414G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SMPD2 antibody (N-term).
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Molecular Weight |
47646 Da
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Cellular Localization |
Antigen Cellular Localization:
Membrane; Multi-pass membrane protein.
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
9-35 aa
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Target |
This SMPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-35 amino acids from the N-terminal region of human SMPD2.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
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Summary |
SMPD2 converts sphingomyelin to ceramide and hydrolyzes 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, FC, E
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Dilution |
WB~~1:1000
FC~~1:10~50
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Synonyms |
Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, Neutral sphingomyelinase, N-SMase, nSMase, SMPD2
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Images |
All lanes : Anti-SMPD2 Antibody (N-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of SMPD2 Antibody (N-term) (Cat. APR13414G) in mouse lung tissue lysates (35ug/lane). SMPD2 (arrow) was detected using the purified Pab.
SMPD2 Antibody (N-term) (Cat. APR13414G) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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