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NSE Antibody (Y25)

Catalog # Availability Size / Price Inquiry
APR08840G 100 μl / $495

NSE Antibody (Y25)

Brand

Leading Biology

Catalog Number

APR08840G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NSE antibody (Y25).

Molecular Weight

47269 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

6-32 aa

Target

This NSE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-32 amino acids from human NSE.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Summary

NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:50~100

Synonyms

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

Images

Western blot analysis of NSE-Y25 Antibody (Cat. APR08840G) in mouse cerebellum tissue and Y79, HepG2, Jurkat, CEM cell line lysates (35ug/lane). NSE (arrow) was detected using the purified Pab.

NSE Antibody (Y25) (Cat. APR08840G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSE Antibody (Y25) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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