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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > ITGA6 Antibody (isoform 2 S1064)

ITGA6 Antibody (isoform 2 S1064)

Catalog # Availability Size / Price Inquiry
APR16915G 100 μl / $495

ITGA6 Antibody (isoform 2 S1064)

Brand

Leading Biology

Catalog Number

APR16915G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ITGA6 antibody (isoform 2 S1064).

Host

Rabbit

Species Reactivity

Human

Immunogen

1043-1072 aa

Target

This ITGA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1043-1072 amino acids from isoform 2 of human ITGA6.

Isotype

Rabbit Ig

UniProt ID

Summary

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, IF, E

Dilution

WB~~1:1000 IF~~1:200 IHC-P~~1:10~50

Synonyms

Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD49f, Integrin alpha-6 heavy chain, Integrin alpha-6 light chain, Processed integrin alpha-6, Alpha6p, ITGA6

Images

Anti-ITGA6 Antibody (isoform 2 S1064) at 1:1000 dilution + SW480 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 119 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of HeLa cells stained with ITGA6 (isoform 2 S1064) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with APR16915G ITGA6 (isoform 2 S1064) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the ITGA6 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000091409).

Western blot analysis of anti-ITGA6 Antibody (isoform 2 S1064) (Cat. APR16915G) in 293 cell line lysates (35ug/lane). ITGA6(arrow) was detected using the purified Pab.

Specification

Quantity

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