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FRAT1 Antibody (C-term)

APR14337G Product Datasheet Protocol

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APR14337G		100 μl / $495

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Leading Biology Inc.

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FRAT1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR14337G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality FRAT1 antibody (C-term).
Molecular Weight	29093 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human
Immunogen	244-274 aa
Target	This FRAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-274 amino acids from the C-terminal region of human FRAT1.
Isotype	Rabbit Ig
GeneID	10023
UniProt ID	Q92837
Function	Positively regulates the Wnt signaling pathway by stabilizing beta-catenin through the association with GSK-3. May play a role in tumor progression and collaborate with PIM1 and MYC in lymphomagenesis.
Summary	FRAT1 belongs to the GSK-3-binding protein family. It may function in tumor progression and in lymphomagenesis.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:10~50
Synonyms	Proto-oncogene FRAT1, Frequently rearranged in advanced T-cell lymphomas 1, FRAT-1, FRAT1
Images	Western blot analysis of lysates from SK-BR-3, K562 cell line (from left to right), using FRAT1 Antibody (C-term)(Cat. APR14337G). APR14337G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FRAT1 antibody (C-term) (Cat. APR14337G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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