We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CRYAB antibody (Center).

20159 Da

Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles

Rabbit

Human, Mouse

84-112 aa

This CRYAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the Central region of human CRYAB.

Rabbit Ig

CRYA2

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.

Crystallins are separated into two classes:taxon-specific, or enzyme, and ubiquitous. The latter classconstitutes the major proteins of vertebrate eye lens and maintainsthe transparency and refractive index of the lens. Since lenscentral fiber cells lose their nuclei during development, thesecrystallins are made and then retained throughout life, making themextremely stable proteins. Mammalian lens crystallins are dividedinto alpha, beta, and gamma families; beta and gamma crystallinsare also considered as a superfamily. Alpha and beta families arefurther divided into acidic and basic groups. Seven protein regionsexist in crystallins: four homologous motifs, a connecting peptide,and N- and C-terminal extensions. Alpha crystallins are composed oftwo gene products: alpha-A and alpha-B, for acidic and basic,respectively. Alpha crystallins can be induced by heat shock andare members of the small heat shock protein (sHSP also known as theHSP20) family. They act as molecular chaperones although they donot renature proteins and release them in the fashion of a truechaperone; instead they hold them in large soluble aggregates.Post-translational modifications decrease the ability to chaperone.These heterogeneous aggregates consist of 30-40 subunits; thealpha-A and alpha-B subunits have a 3:1 ratio, respectively. Twoadditional functions of alpha crystallins are an autokinaseactivity and participation in the intracellular architecture.Alpha-A and alpha-B gene products are differentially expressed;alpha-A is preferentially restricted to the lens and alpha-B isexpressed widely in many tissues and organs. Elevated expression ofalpha-B crystallin occurs in many neurological diseases; a missensemutation cosegregated in a family with a desmin-related myopathy.

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

WB, IHC-P, E

WB~~1:1000 IHC-P~~1:10~50

Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, CRYAB, CRYA2

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