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TUBA4A Antibody (C-term)

APR13862G Product Datasheet Protocol

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APR13862G		100 μl / $495

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Leading Biology Inc.

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TUBA4A Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR13862G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TUBA4A antibody (C-term).
Molecular Weight	49924 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytoskeleton.
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	417-446 aa
Target	This TUBA4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 417-446 amino acids from the C-terminal region of human TUBA4A.
Isotype	Rabbit Ig
Symbol	TUBA1
GeneID	7277
UniProt ID	P68366
Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Summary	Microtubules of the eukaryotic cytoskeleton performessential and diverse functions and are composed of a heterodimerof alpha and beta tubulin. The genes encoding these microtubuleconstituents are part of the tubulin superfamily, which is composedof six distinct families. Genes from the alpha, beta and gammatubulin families are found in all eukaryotes. The alpha and betatubulins represent the major components of microtubules, whilegamma tubulin plays a critical role in the nucleation ofmicrotubule assembly. There are multiple alpha and beta tubulingenes and they are highly conserved among and between species. Thisgene encodes an alpha tubulin that is a highly conserved homolog ofa rat testis-specific alpha tubulin.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:10~50
Synonyms	Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A, TUBA1
Images	All lanes : Anti-TUBA4A Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST. TUBA4A Antibody (C-term) (Cat. APR13862G) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the TUBA4A antibody detected the TUBA4A protein (arrow). TUBA4A Antibody (C-term) (Cat. APR13862G)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TUBA4A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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