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PI15 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR12880G 100 μl / $495

PI15 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR12880G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PI15 antibody (N-term).

Molecular Weight

29065 Da

Cellular Localization

Antigen Cellular Localization: Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

34-63 aa

Target

This PI15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human PI15.

Isotype

Rabbit Ig

Symbol

CRISP8, P25TI

GeneID

UniProt ID

Function

Serine protease inhibitor which displays weak inhibitory activity against trypsin.

Summary

This gene encodes a trypsin inhibitor. The protein sharessimilarity to insect venom allergens, mammalian testis-specificproteins and plant pathogenesis-related proteins. It is frequentlyexpressed in human neuroblastoma and glioblastoma cell lines, andthus may play a role in the central nervous system. [provided byRefSeq].

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50

Synonyms

Peptidase inhibitor 15, PI-15, 25 kDa trypsin inhibitor, p25TI, Cysteine-rich secretory protein 8, CRISP-8, SugarCrisp, PI15, CRISP8, P25TI

Images

All lanes : Anti-PI15 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

PI15 Antibody (N-term) (Cat. APR12880G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).

PI15 Antibody (N-term) (Cat. APR12880G)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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