> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > PI15 Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APR12880G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PI15 antibody (N-term).
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Molecular Weight |
29065 Da
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Cellular Localization |
Antigen Cellular Localization:
Secreted.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
34-63 aa
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Target |
This PI15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human PI15.
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Isotype |
Rabbit Ig
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Symbol |
CRISP8, P25TI
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GeneID |
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UniProt ID |
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Function |
Serine protease inhibitor which displays weak inhibitory activity against trypsin.
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Summary |
This gene encodes a trypsin inhibitor. The protein sharessimilarity to insect venom allergens, mammalian testis-specificproteins and plant pathogenesis-related proteins. It is frequentlyexpressed in human neuroblastoma and glioblastoma cell lines, andthus may play a role in the central nervous system. [provided byRefSeq].
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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Synonyms |
Peptidase inhibitor 15, PI-15, 25 kDa trypsin inhibitor, p25TI, Cysteine-rich secretory protein 8, CRISP-8, SugarCrisp, PI15, CRISP8, P25TI
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Images |
All lanes : Anti-PI15 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PI15 Antibody (N-term) (Cat. APR12880G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).
PI15 Antibody (N-term) (Cat. APR12880G)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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