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SLC16A10 Antibody (C-term)

AMM07835G Product Datasheet Protocol

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AMM07835G		100 μl / $495

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Leading Biology Inc.

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SLC16A10 Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM07835G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SLC16A10 antibody (C-term).
Molecular Weight	55493 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein. Basolateral cell membrane
Host	Rabbit
Species Reactivity	Human
Immunogen	301-329 aa
Target	This SLC16A10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-329 amino acids from the C-terminal region of human SLC16A10.
Isotype	Rabbit Ig
Symbol	MCT10, TAT1
GeneID	117247
UniProt ID	Q8TF71
Function	Sodium-independent transporter that mediates the update of aromatic acid. Can function as a net efflux pathway for aromatic amino acids in the basosolateral epithelial cells (By similarity).
Summary	SLC16A10 is a member of a family of plasma membrane aminoacid transporters that mediate the Na(+)-independent transport ofaromatic amino acids across the plasma membrane.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	IHC-P~~1:10~50 WB~~1:1000 FC~~1:10~50
Synonyms	Monocarboxylate transporter 10, MCT 10, Aromatic amino acid transporter 1, Solute carrier family 16 member 10, T-type amino acid transporter 1, SLC16A10, MCT10, TAT1
Images	AMM07835G staining SLC16A10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-SLC16A10 Antibody (C-term) at 1:2000 dilution Lane 1: human skeletal muscle lysates Lane 2: CCRF-CEM whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST. SLC16A10 Antibody (C-term) (Cat. AMM07835G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC16A10 antibody detected the SLC16A10 protein (arrow).
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