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KLC2 Antibody (N-term)

APR14350G Product Datasheet Protocol

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APR14350G		100 μl / $495

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Leading Biology Inc.

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KLC2 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR14350G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KLC2 antibody (N-term).
Molecular Weight	68935 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm, cytoskeleton.
Host	Rabbit
Species Reactivity	Human
Immunogen	128-156 aa
Target	This KLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-156 amino acids from the N-terminal region of human KLC2.
Isotype	Rabbit Ig
GeneID	64837
UniProt ID	Q9H0B6
Function	Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity (By similarity).
Summary	Kinesin is a molecular motor that generates ATP-dependentmovement of vesicles and organelles along microtubules. Kinesinconsists of 2 light chains, such as KLC2, and 2 heavy chains (seeKIF5B; MIM 602809) in a 1:1 stoichiometric ratio (Rahman et al.,1998 [PubMed 9624122]).
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
Synonyms	Kinesin light chain 2, KLC 2, KLC2
Images	Western blot analysis of lysate from MCF-7 cell line, using KLC2 Antibody (N-term)(Cat. APR14350G). APR14350G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. KLC2 Antibody (N-term) (Cat. APR14350G)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KLC2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. KLC2 Antibody (N-term) (Cat. APR14350G) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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