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MSI1 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
AMM06492G 100 μl / $495

MSI1 Antibody (N-term)

Brand

Leading Biology

Catalog Number

AMM06492G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MSI1 antibody (N-term).

Molecular Weight

39125 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus

Host

Rabbit

Species Reactivity

Human

Immunogen

67-93 aa

Target

This MSI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the N-terminal region of human MSI1.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

Summary

This gene encodes a protein containing two conservedtandem RNA recognition motifs. Similar proteins in other speciesfunction as RNA-binding proteins and play central roles inposttranscriptional gene regulation. Expression of this gene hasbeen correlated with the grade of the malignancy and proliferativeactivity in gliomas and melanomas. A pseudogene for this gene islocated on chromosome 11q13.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Synonyms

RNA-binding protein Musashi homolog 1, Musashi-1, MSI1

Images

Western blot analysis of lysate from SH?SY5Y cell line, using MSI1 Antibody (N-term)(Cat. AMM06492G). AMM06492G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

MSI1 Antibody (N-term) (Cat. AMM06492G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MSI1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

MSI1 Antibody (N-term) (Cat. AMM06492G) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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