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AMACR Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APG01835G 100 μl / $495

AMACR Antibody (C-term)

Brand

Leading Biology

Catalog Number

APG01835G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality AMACR antibody (C-term).

Molecular Weight

42387 Da

Cellular Localization

Antigen Cellular Localization: Peroxisome. Mitochondrion

Host

Rabbit

Species Reactivity

Human

Immunogen

323-351 aa

Target

This AMACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the C-terminal region of human AMACR.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.

Summary

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, IF, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50

Synonyms

Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR

Images

All lanes : Anti-AMACR Antibody (C-term) at 1:1000 dilution Lane 1: human kidney lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

AMACR Antibody (C-term) (Cat. APG01835G) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the AMACR antibody detected the AMACR protein (arrow).

AMACR antibody (C-term) (Cat. APG01835G) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMACR antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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