> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > AMACR Antibody (C-term)Brand |
Leading Biology | Catalog Number |
APG01835G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality AMACR antibody (C-term).
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Molecular Weight |
42387 Da
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Cellular Localization |
Antigen Cellular Localization:
Peroxisome. Mitochondrion
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
323-351 aa
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Target |
This AMACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the C-terminal region of human AMACR.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
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Summary |
This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, IF, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
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Synonyms |
Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR
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Images |
All lanes : Anti-AMACR Antibody (C-term) at 1:1000 dilution Lane 1: human kidney lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AMACR Antibody (C-term) (Cat. APG01835G) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the AMACR antibody detected the AMACR protein (arrow).
AMACR antibody (C-term) (Cat. APG01835G) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMACR antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Leading Biology Inc.
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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