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SOD1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR10851G 100 μl / $545

SOD1 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR10851G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SOD1 antibody (Center).

Molecular Weight

15936 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria

Host

Rabbit

Species Reactivity

Human

Immunogen

55-84 aa

Target

This SOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human SOD1.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Summary

SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, IF, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50

Synonyms

Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1, SOD1

Images

Western blot analysis of SOD1 Antibody (Center) (Cat. APR10851G) in Jurkat cell line lysates (35ug/lane). SOD1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOD1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

SOD1 Antibody (Center) (Cat. APR10851G) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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