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ADRBK1 Antibody (C-term)

APR14068G Product Datasheet Protocol

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APR14068G		100 μl / $545

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Leading Biology Inc.

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ADRBK1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR14068G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ADRBK1 antibody (C-term).
Molecular Weight	79574 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm {ECO:0000250\|UniProtKB:P21146}. Cell membrane {ECO:0000250\|UniProtKB:P21146}
Host	Rabbit
Species Reactivity	Human
Immunogen	633-660 aa
Target	This ADRBK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633-660 amino acids from the C-terminal region of human ADRBK1.
Isotype	Rabbit Ig
Symbol	BARK, BARK1, GRK2
GeneID	156
UniProt ID	P25098
Function	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Summary	Beta-adrenergic receptor kinase (ADRBK1), also known as GRK2, phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. ADRBK1 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Heart failure is accompanied by severely impaired beta-adrenergic receptor (beta-AR) function. An important mechanism for the rapid desensitization of beta-AR function is agonist-stimulated receptor phosphorylation by the beta-AR kinase (beta-ARK1), an enzyme known to be elevated in failing human heart tissue. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:50~100
Synonyms	Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2
Images	Western blot analysis of anti-GRK2 C-term Pab (Cat. APR14068G) in Ramos cell lysate. GRK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRK2 Antibody (C-term) (Cat. APR14068G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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