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(Mouse) Dpf2 Antibody (N-term)

APR06934G Product Datasheet Protocol

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APR06934G		100 μl / $495

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Leading Biology Inc.

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(Mouse) Dpf2 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR06934G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Dpf2 Antibody (N-term).
Molecular Weight	M=44;H=44,24 KDa
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Nucleus.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	99-133 aa
Target	This mouse Dpf2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 99-133 amino acids from the N-terminal region of mouse Dpf2.
Symbol	Baf45d, Req, Ubid4
GeneID	19708
UniProt ID	Q61103
Function	May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Summary	May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB
Dilution	IHC~~1:25 WB~~1:2000
Images	APR06934G staining (Mouse) Dpf2 in mouse testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. APR06934G staining (Mouse) Dpf2 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-Dpf2 Antibody (N-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: mouse thymus lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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