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POU2AF1 Antibody (C-Term)

Catalog # Availability Size / Price Inquiry
APG00673G 0.1mg / $495

POU2AF1 Antibody (C-Term)

Brand

Leading Biology

Catalog Number

APG00673G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality POU2AF1 Antibody (C-Term).

Molecular Weight

27436 Da

Cellular Localization

Antigen Cellular Localization: Nucleus.

Host

Goat

Species Reactivity

Human

Isotype

IgG

Symbol

OBF1

GeneID

UniProt ID

Function

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Form

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC, E

Images

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with APG00673G(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with APG00673G(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5 μg/ml.

Specification

Quantity

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